Mao Ayuth was born on 8 July 1944 in Kompong Cham in Cambodia. He was separated from his family in 1963 and went to live with his uncle who is also a famous Cambodian author by the name of Peou Sipho. It was during this time at Sisowath High School that his interest in writing developed. From 1963 to 1965 he underwent training as a script writer. Upon completion, he joined TVRK as Floor Director and eventually was promoted to Program Director in 1975. It was during this year that he and his countrymen were forced to leave Phnom Penh by the Pol Pot regime. During these four years where he felt like "a prisoner", he continued to produce literary works but at a slower pace. In 1979 the returned to the capital city and joined the Ministry of Culture and Information as Deputy Director of the Cinema Department until 1992 when he became Deputy General Director of the National & Television of Kampuchea. In 1994 he was appointed General Director of the National Television of Cambodia.

Mao Ayuth is a producer and script writer of sentimental stories in film and drama. He is also a novelist focusing on the reality of society and its lifestyle. Moreover, he also writes lyrical, epistle and satirical poetry. Mao Ayuth has been a member of the Khmers Writers Association since 1963 and has also been invited to be a member of the jury at the Documentary Film Festival held in Leipzig, Germany in 1981 and also at the International Teenagers Film Festival in Moscow in both 1982 and 1984.

During his career, he has been awarded the Bronze Medal for Labour by the Royal Government of Cambodia in 1998 and the Gold Medal in 2001.